answer:Introduction The Chinese Socialist System with Chinese Characteristics, adopted in the 1970s, has had a significant impact on the world, particularly in the realm of economics. Under the guidance of this system, China has undergone a rapid transformation, becoming a global economic powerhouse. This paper explores the impacts of the Chinese Socialist System with Chinese Characteristics on the rise of China as a dominant economic power in the world. The paper examines the policies, strategies, and initiatives that have been implemented under this system and evaluates their impacts on China's economic growth and global economic influence. Economic Growth The Chinese Socialist System with Chinese Characteristics has played a critical role in China's economic transformation, moving the economy from a centrally planned system to a market-oriented one. The system has introduced policies that promote private enterprise, encourage foreign investment, and foster innovation and technological advancement. These policies have led to significant economic growth, with China becoming the world's second-largest economy behind the United States. The Chinese economy has also become a critical driving force in the global economy, accounting for approximately 16% of global GDP (IMF, 2020). The country's economic expansion has created new opportunities for global trade and investment, contributing to increased economic interdependence between China and other countries. Global businesses and investors have recognized the potential of China's market size and growth prospects, leading to increased foreign investment in the country. This has further fueled China's economic growth and helped to drive the country's rise as a dominant economic power in the world. Technology and Innovation The Chinese Socialist System with Chinese Characteristics has been instrumental in fostering the growth of science and technology and has provided a conducive environment for innovation by implementing policies that promote research and development. This has led to China becoming a leader in various technological sectors, such as telecommunication, artificial intelligence, and robotics, among others. The country's technological advancements have led to the integration of the global economy, as the country has become a vital player in global value chains, particularly in manufacturing. As a result of China's technological advancements, Chinese high-tech companies have become global leaders, challenging traditional Western dominance in various sectors. For example, Huawei, a Chinese technology company, has become the world's largest telecommunications equipment maker and a global leader in the 5G technology sector. The growth of Chinese high-tech companies has had ripple effects on the broader economy, contributing to job creation, economic growth, and increased productivity. Global Influence The rise of China as a dominant economic power has challenged the traditional hegemony of Western powers, particularly the United States. China's increasing influence and power on the world stage have had implications for global governance, trade, and security, as countries have sought to adjust to China's growing economic and political influence. Moreover, China's socialist approach has been influential in shaping the global discourse on governance, especially in developing countries. The Chinese model, which combines economic liberalism with strong state intervention, has been increasingly attractive to developing countries that are seeking to reconstruct their economies or reduce income inequality. This has led to China offering assistance to developing countries, particularly on infrastructure development, as part of its Belt and Road Initiative. This approach has helped the Chinese government gain influence in developing countries and challenge the traditional influence of Western countries. However, the Chinese Socialist System with Chinese Characteristics has also faced criticism for its restrictions on individual freedom and human rights. The system has been accused of suppressing dissent and engaging in human rights violations, leading to tensions with Western countries and criticism from human rights organizations. Conclusion In conclusion, the Chinese Socialist System with Chinese Characteristics has been instrumental in shaping the rise of China as a dominant economic power in the world. The system's policies, strategies, and initiatives have led to significant economic growth, technological advancements, and increased global influence. However, the system's approach to governance has faced criticism for its limitations on individual freedom and human rights. As China continues to grow as a global power, the impacts of the socialist system on the world will likely continue to evolve and shape the global landscape in unprecedented ways.

answer:引言 中国特色社会主义制度于上世纪70年代采取，对世界产生了显著影响，特别是在经济领域。在这一体系的指导下，中国经历了快速转型，成为全球经济强国。本文探讨中国特色社会主义制度对中国崛起为世界主要经济大国的影响。文章审视了在此体系下实施的政策、战略和举措，并评估它们对中国经济增长和全球经济影响的影响。 经济增长 中国特色社会主义制度在中国的经济转型中发挥了关键作用，将经济从计划经济体制转变为市场导向体制。该体系提出了促进私营企业、鼓励外国投资和促进创新和技术进步的政策。这些政策已带来了显著的经济增长，中国已成为仅次于美国的世界第二大经济体。 中国经济也已成为全球经济的重要推动力，占全球GDP约16％（IMF，2020年）。该国的经济扩张为全球贸易和投资创造了新机遇，促进了中国与其他国家之间的增加经济相互依赖性。全球企业和投资者已经认识到中国市场规模和增长前景的潜力，这导致了对中国的外国投资增加。这进一步推动了中国的经济增长，帮助该国崛起为世界主要经济大国。 技术与创新 中国特色社会主义制度在促进科技增长方面起着重要作用，并通过实施促进研究和发展的政策为创新提供了有利环境。这导致中国成为各种技术领域的领军者，例如电信、人工智能和机器人等。该国的技术进步已导致全球经济的融合，因为该国已成为全球价值链的重要参与者，特别是在制造业方面。 由于中国的技术进步，中国的高科技公司已成为全球领导者，在各个领域挑战传统的西方优势。例如，中国技术公司华为已成为全球最大的电信设备制造商，也是全球5G技术领域的全球领导者。中国高科技公司的增长对整个经济产生了波及效应，促进了创造就业、经济增长和提高生产率。 全球影响 中国作为一个主要经济大国的崛起挑战了传统的西方大国，特别是美国的传统霸权。中国在世界舞台上

answer:1. 消化结束后，将样品转移至离心管中，并用力震荡使肝脏降解破碎。随后使用70μm滤网过滤，使用生理盐水冲洗，将滤液转移至15ml无菌离心管中，加入足量生理盐水使其达到15ml刻度线。在离体肝细胞处理过程中，应尽量在冰上操作。 2. 将离心管放入低温离心机中，设置转速为800rpm，温度为4℃，离心3min。去除上层液体后，使用细胞培养液重悬细胞，并将其转移至15ml无菌离心管中重复上述步骤。随后对样品进行不同离心条件下的离心，分别为800rpm、3min、4℃（加速度为0）；800rpm、2min、4℃（加速度为0）；500rpm、2min、4℃（加速度为0）。由于肝实质细胞重量最大，因此最后留在离心管底部的应为肝细胞。 3. 将细胞培养液重悬肝细胞，并取20μl转入细胞计数板中，随后将其移至六孔板中培养，使细胞密度为1×106。在显微镜下观察，肝实质细胞呈双核结构，边缘圆润，结构特征清晰明显。

answer:在六孔板中培养肝原代细胞，分别设置KYNA对照组、低剂量组和高剂量组。KYNA母液使用William's Medium E培养基配制，浓度为10 mmol/l。各组的给药浓度分别为低剂量组（10 μmol/l）和高剂量组（100 μmol/l），处理时间为48小时。处理结束后，向每孔加入1 ml的TRIzon用于取样收集细胞，然后进行qPCR实验。